2022
Novel Multilocus Sequence Typing and Global Sequence Clustering Schemes for Characterizing the Population Diversity of Streptococcus mitis
Kalizang’oma A, Kwambana-Adams B, Chan J, Viswanath A, Gori A, Richard D, Jolley K, Lees J, Goldblatt D, Beleza S, Bentley S, Heyderman R, Chaguza C. Novel Multilocus Sequence Typing and Global Sequence Clustering Schemes for Characterizing the Population Diversity of Streptococcus mitis. Journal Of Clinical Microbiology 2022, 61: e00802-22. PMID: 36515506, PMCID: PMC9879099, DOI: 10.1128/jcm.00802-22.Peer-Reviewed Original ResearchConceptsSequence clustersPathogenic streptococcal speciesMultilocus sequence typing (MLST) schemePopulation diversitySingle-nucleotide polymorphism distancesSequence typesSequence typing schemeUnique sequence typesGenetic diversityPopulation structureBacterial population diversityMolecular typing toolsS. mitisHousekeeping genesSequence fragmentsMLST schemeDiversityOpportunistic pathogenStreptococcus mitisStreptococcal speciesTyping toolSpeciesNew insightsTyping schemeMultilocus sequence typingEvaluation of Dried Blood and Cerebrospinal Fluid Filter Paper Spots for Storing and Transporting Clinical Material for the Molecular Diagnosis of Invasive Meningococcal Disease
Kwambana-Adams B, Clark S, Tay N, Agbla S, Chaguza C, Kagucia E, Borrow R, Heyderman R. Evaluation of Dried Blood and Cerebrospinal Fluid Filter Paper Spots for Storing and Transporting Clinical Material for the Molecular Diagnosis of Invasive Meningococcal Disease. International Journal Of Molecular Sciences 2022, 23: 11879. PMID: 36233182, PMCID: PMC9569512, DOI: 10.3390/ijms231911879.Peer-Reviewed Original ResearchConceptsInvasive meningococcal diseaseMeningococcal diseaseNeisseria meningitidis infectionResource-limited settingsLow-resource settingsFilter paper spotsMeningococcal infectionReal-time PCRCerebrospinal fluidClinical specimensSubset of specimensBlood spotsSpot assayAntimicrobial resistanceBloodMolecular diagnosisClonal complexesClinical materialPaper spotsWhole-genome sequencingDBSInfectionSequence typesDiseaseDiagnosis
2020
Hypervirulent pneumococcal serotype 1 harbours two pneumolysin variants with differential haemolytic activity
Panagiotou S, Chaguza C, Yahya R, Audshasai T, Baltazar M, Ressel L, Khandaker S, Alsahag M, Mitchell TJ, Prudhomme M, Kadioglu A, Yang M. Hypervirulent pneumococcal serotype 1 harbours two pneumolysin variants with differential haemolytic activity. Scientific Reports 2020, 10: 17313. PMID: 33057054, PMCID: PMC7560715, DOI: 10.1038/s41598-020-73454-w.Peer-Reviewed Original ResearchConceptsPneumococcal serotype 1Invasive pneumococcal diseaseSerotype 1Haemolytic activityVivo mouse modelDetectable haemolytic activityNeutrophil-mediated phagocytosisPneumococcal diseaseCommon causeInfection doseHuman reservoirMouse modelRapid onsetStreptococcus pneumoniaeDisease symptomsLethal doseClonal typesVirulence propertiesGlobal pathogenST306IgA proteaseSymptomsSequence typesMiceDose