2023
Saliva as an alternative sample type for detection of pneumococcal carriage in young children
Wyllie A, Rots N, Wijmenga-Monsuur A, van Houten M, Sanders E, Trzciński K. Saliva as an alternative sample type for detection of pneumococcal carriage in young children. Microbiology 2023, 169: 001394. PMID: 37819029, PMCID: PMC10634364, DOI: 10.1099/mic.0.001394.Peer-Reviewed Original ResearchConceptsNasopharyngeal swabsCarriage detectionPneumococcal carriageSaliva samplesSubset of serotypesOlder age groupsConventional cultureAlternative sample typePneumococcal colonizationPneumococcal detectionPolymicrobial growthAge groupsStudy participantsSaliva samplingSwabsDiagnostic culturesGold standardLongitudinal studyYoung childrenSalivaCarriageChildrenInfantsMolecular detection methodsPneumococciPersistence of Pneumococcal Carriage among Older Adults in the Community despite COVID-19 Mitigation Measures
Wyllie A, Mbodj S, Thammavongsa D, Hislop M, Yolda-Carr D, Waghela P, Nakahata M, Stahlfeld A, Vega N, York A, Allicock O, Wilkins G, Ouyang A, Siqueiros L, Strong Y, Anastasio K, Alexander-Parrish R, Arguedas A, Gessner B, Weinberger D. Persistence of Pneumococcal Carriage among Older Adults in the Community despite COVID-19 Mitigation Measures. Microbiology Spectrum 2023, 11: e04879-22. PMID: 37036377, PMCID: PMC10269788, DOI: 10.1128/spectrum.04879-22.Peer-Reviewed Original ResearchConceptsInvasive pneumococcal diseasePneumococcal diseaseSchool-aged childrenOlder adultsPneumococcal carriageSaliva samplesCOVID-19 pandemicRate of carriageCOVID-19-related disruptionsUpper respiratory tractRegular contactNew Haven areaFirst yearCarriage prevalencePre-pandemic levelsMedical historyRespiratory tractHigh prevalenceStreptococcus pneumoniaeCOVID-19 mitigation measuresStudy participantsPneumococciPrevalenceDiseaseCarriage
2022
Pneumococcal genetic variability in age-dependent bacterial carriage
Kremer P, Ferwerda B, Bootsma HJ, Rots NY, Wijmenga-Monsuur AJ, Sanders E, Trzciński K, Wyllie AL, Turner P, van der Ende A, Brouwer MC, Bentley SD, van de Beek D, Lees JA. Pneumococcal genetic variability in age-dependent bacterial carriage. ELife 2022, 11: e69244. PMID: 35881438, PMCID: PMC9395192, DOI: 10.7554/elife.69244.Peer-Reviewed Original ResearchConceptsSecond cohortImproved vaccine formulationsFirst cohortFuture vaccination strategiesSingle nucleotide polymorphismsHost immune factorsAge-specific differencesPneumococcal carriageImmune factorsVaccination strategiesBacterial carriageCarriage studiesVaccine formulationsCohortGenetic factorsAdult hostsSerotypesHost ageAdultsCarriageChildrenGenetic backgroundWeak evidenceInfantsPopulation
2020
Serotype Patterns of Pneumococcal Disease in Adults Are Correlated With Carriage Patterns in Older Children
Wyllie AL, Warren JL, Regev-Yochay G, Givon-Lavi N, Dagan R, Weinberger DM. Serotype Patterns of Pneumococcal Disease in Adults Are Correlated With Carriage Patterns in Older Children. Clinical Infectious Diseases 2020, 72: e768-e775. PMID: 32989457, PMCID: PMC8315131, DOI: 10.1093/cid/ciaa1480.Peer-Reviewed Original ResearchConceptsInvasive pneumococcal diseaseSerotype-specific invasive pneumococcal diseaseDisease patternsOlder childrenPneumococcal diseasePneumococcal carriageCarriage prevalenceSerotype patternsCause of IPDIPD dataSerotype-specific carriage prevalenceAge groupingsDifferent age groupsComorbidity statusDisease burdenVaccination strategiesAge-related patternsCarriage dataCarriage patternsDifferent age categoriesAge groupsSpecific serotypesYoung adultsAdultsAge categories
2018
Exploring Immune Development in Infants With Moderate to Severe Atopic Dermatitis
Hulshof L, Overbeek SA, Wyllie AL, Chu MLJN, Bogaert D, de Jager W, Knippels LMJ, Sanders EAM, van Aalderen WMC, Garssen J, Land B, Sprikkelman AB, Group T, Blauw A, Dontje B, Duijvestein Y, de Boom W, Groot I, Boks M, van Kooyk Y, Fandri D, Hijnen D, Middelkamp-Hup M, Papi B, Roelofs M, Rijnierse A, Veening D, Support C, Prakken B, Tusscher G, Tupker R, Willemsen L. Exploring Immune Development in Infants With Moderate to Severe Atopic Dermatitis. Frontiers In Immunology 2018, 9: 630. PMID: 29966024, PMCID: PMC5884950, DOI: 10.3389/fimmu.2018.00630.Peer-Reviewed Original ResearchConceptsSevere atopic dermatitisAtopic dermatitisDietary interventionAD severityCommon chronic inflammatory skin diseaseT helper cell type 2Understanding of ADChronic inflammatory skin diseaseCC chemokine ligand 17CXC chemokine ligand 9Serum chemokine levelsSpecific IgE levelsInflammatory skin diseaseChemokine ligand 17Cell type 2Dietary intervention studyWhey-based formulaChemokine ligand 9Post-intervention samplesMonths of ageCCL22 chemokinesTh1 chemokinesTh2 chemokinesChemokine levelsM-16V
2016
Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines
Wyllie AL, Wijmenga-Monsuur AJ, van Houten MA, Bosch AATM, Groot JA, van Engelsdorp Gastelaars J, Bruin JP, Bogaert D, Rots NY, Sanders EAM, Trzciński K. Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines. Scientific Reports 2016, 6: 23809. PMID: 27046258, PMCID: PMC4820691, DOI: 10.1038/srep23809.Peer-Reviewed Original ResearchConceptsPneumococcal conjugate vaccinePolysaccharide pneumococcal vaccineVaccine-targeted serotypesStreptococcus pneumoniae carriageCross-sectional surveillanceVaccine serotypes 4Number of serotypesPneumococcal surveillancePneumococcal vaccinePneumoniae carriageUnvaccinated individualsConjugate vaccineNasopharyngeal carriagePneumococcal diseaseVaccine effectsNasopharyngeal samplesConventional diagnostic methodsSurveillance studyS. pneumoniaeStreptococcus pneumoniaeSerotype detectionMolecular surveillanceHidden circulationSerotypesDiagnostic methodsDevelopment of the Nasopharyngeal Microbiota in Infants with Cystic Fibrosis
Prevaes SM, de Winter-de Groot KM, Janssens HM, de Steenhuijsen Piters WA, Tramper-Stranders GA, Wyllie AL, Hasrat R, Tiddens HA, van Westreenen M, van der Ent CK, Sanders EA, Bogaert D. Development of the Nasopharyngeal Microbiota in Infants with Cystic Fibrosis. American Journal Of Respiratory And Critical Care Medicine 2016, 193: 504-515. PMID: 26492486, DOI: 10.1164/rccm.201509-1759oc.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBurkholderiaBurkholderia InfectionsCarrier StateCase-Control StudiesCohort StudiesCorynebacteriumCorynebacterium InfectionsCystic FibrosisDNA, BacterialEnterobacteriaceaeEnterobacteriaceae InfectionsFemaleHaemophilus InfectionsHaemophilus influenzaeHumansInfantInfant, NewbornMaleMicrobiotaMoraxellaMoraxellaceae InfectionsNasopharynxProspective StudiesReal-Time Polymerase Chain ReactionRNA, Ribosomal, 16SStaphylococcal InfectionsStaphylococcus aureusStreptococcal InfectionsStreptococcus mitisConceptsHealthy control subjectsControl subjectsMonths of lifeMonths of ageCystic fibrosisAntibiotic useMicrobiota profilesAge-matched healthy control subjectsEarly structural lung diseaseNasopharyngeal microbiota profilesNasopharynx of infantsProspective cohort studyStructural lung diseaseTime of diagnosisMoraxella sppPotential respiratory pathogensRespiratory symptomsAntibiotic therapyCohort studyPulmonary infectionLung diseaseNasopharyngeal microbiotaRespiratory pathogensHaemophilus influenzaeNasopharynx samples