2019
Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis
Lees JA, Ferwerda B, Kremer PHC, Wheeler NE, Serón MV, Croucher NJ, Gladstone RA, Bootsma HJ, Rots NY, Wijmega-Monsuur AJ, Sanders EAM, Trzciński K, Wyllie AL, Zwinderman AH, van den Berg LH, van Rheenen W, Veldink JH, Harboe ZB, Lundbo LF, de Groot LCPGM, van Schoor NM, van der Velde N, Ängquist LH, Sørensen TIA, Nohr EA, Mentzer AJ, Mills TC, Knight JC, du Plessis M, Nzenze S, Weiser JN, Parkhill J, Madhi S, Benfield T, von Gottberg A, van der Ende A, Brouwer MC, Barrett JC, Bentley SD, van de Beek D. Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis. Nature Communications 2019, 10: 2176. PMID: 31092817, PMCID: PMC6520353, DOI: 10.1038/s41467-019-09976-3.Peer-Reviewed Original ResearchConceptsGenetic variationGenome-wide association studiesCommon nasopharyngeal colonizerPathogen genomesHalf of variationPneumococcal factorsPneumococcal genesAssociation studiesJoint sequencingHuman variationInvasive potentialLife-threatening invasive diseasesGenesPathogensInteraction analysisInvasivenessGenomeZmpDGeneticsSequencingColonizersHumansVariationHostSusceptibility
2017
Sequencing of the variable region of rpsB to discriminate between Streptococcus pneumoniae and other streptococcal species
Wyllie AL, Pannekoek Y, Bovenkerk S, van Engelsdorp Gastelaars J, Ferwerda B, van de Beek D, Sanders EAM, Trzciński K, van der Ende A. Sequencing of the variable region of rpsB to discriminate between Streptococcus pneumoniae and other streptococcal species. Open Biology 2017, 7: 170074. PMID: 28931649, PMCID: PMC5627049, DOI: 10.1098/rsob.170074.Peer-Reviewed Original ResearchConceptsSevere streptococcal diseaseUpper respiratory tractHuman upper respiratory tractSerotype-specific assaysMolecular assaysStreptococcal speciesNon-typeable pneumococciStreptococcal diseaseAsymptomatic individualsFalse-positive resultsRespiratory tractPneumococcal isolatesCarriage isolatesStreptococcal isolatesStreptococcus pneumoniaeLack of specificityStreptococciPositive resultsPneumococciDiseaseCarriageAssaysIsolatesVast majorityMeningitis
2014
Streptococcus pneumoniae in Saliva of Dutch Primary School Children
Wyllie AL, Chu ML, Schellens MH, van Engelsdorp Gastelaars J, Jansen MD, van der Ende A, Bogaert D, Sanders EA, Trzciński K. Streptococcus pneumoniae in Saliva of Dutch Primary School Children. PLOS ONE 2014, 9: e102045. PMID: 25013895, PMCID: PMC4094488, DOI: 10.1371/journal.pone.0102045.Peer-Reviewed Original ResearchConceptsPneumoniae carriagePneumococcal carriagePneumococcal strainsMultiple pneumococcal strainsStreptococcus pneumoniae carriageS. pneumoniae carriageS. pneumoniae strainsNumber of serotypesDutch primary school childrenNasopharyngeal samplingSchool-aged childrenPneumococcal detectionSurveillance studyS. pneumoniaePneumoniae strainsStreptococcus pneumoniaeSerotype composition