2023
Pooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2
Allicock O, Yolda-Carr D, Todd J, Wyllie A. Pooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2. Scientific Reports 2023, 13: 7426. PMID: 37156888, PMCID: PMC10165292, DOI: 10.1038/s41598-023-34662-2.Peer-Reviewed Original ResearchConceptsSARS-CoV-2RT-qPCR assaysSARS-CoV-2 testingVirus-infected individualsAged care facilitiesSelf-collected salivaSARS-CoV-2 spreadActionable time frameAsymptomatic individualsTest turnaround timeHealthcare workersGeneral populationCare facilitiesTest availabilityWeekly testingFree testingClinical salivaPositive agreementClinical laboratoriesSalivaCT valuesSuch testingTesting workflowIndividual testingTesting protocol
2021
Implementation of a pooled surveillance testing program for asymptomatic SARS-CoV-2 infections in K-12 schools and universities
Mendoza RP, Bi C, Cheng HT, Gabutan E, Pagapas G, Khan N, Hoxie H, Hanna S, Holmes K, Gao N, Lewis R, Wang H, Neumann D, Chan A, Takizawa M, Lowe J, Chen X, Kelly B, Asif A, Barnes K, Khan N, May B, Chowdhury T, Pollonini G, Gouda N, Guy C, Gordon C, Ayoluwa N, Colon E, Miller-Medzon N, Jones S, Hossain R, Dodson A, Weng M, McGaskey M, Vasileva A, Lincoln AE, Sikka R, Wyllie AL, Berke EM, Libien J, Pincus M, Premsrirut PK. Implementation of a pooled surveillance testing program for asymptomatic SARS-CoV-2 infections in K-12 schools and universities. EClinicalMedicine 2021, 38: 101028. PMID: 34308321, PMCID: PMC8286123, DOI: 10.1016/j.eclinm.2021.101028.Peer-Reviewed Original ResearchSARS-CoV-2School transmissionAsymptomatic SARS-CoV-2 infectionRoutine COVID-19 testingSARS-CoV-2 infectionOverall viral burdenCOVID-19 testingViral burdenNasopharyngeal swabsSaliva specimensPrevention protocolsPCR testingKappa agreementWeekly testingCost-effective strategyPooled testingSaliva analysisMolecular assaysSurveillance methodsSafety protocolsDescriptive statisticsSchool closuresTestingStrong evidenceInfection