2001
Development and Characterization of a Molecular Viability Assay for Pneumocystis carinii f sp hominis
Maher N, Vermund S, Welsh D, Dillon H, Awooda A, Unnasch T. Development and Characterization of a Molecular Viability Assay for Pneumocystis carinii f sp hominis. The Journal Of Infectious Diseases 2001, 183: 1825-1827. PMID: 11372040, DOI: 10.1086/320738.Peer-Reviewed Original ResearchConceptsPneumocystis carinii pneumoniaHuman immunodeficiency virus-infected personsP. cariniiCommon opportunistic infectionVirus-infected personsCarinii pneumoniaOpportunistic infectionsPerson transmissionHuman P. cariniiClinical specimensTransmission dynamicsCariniiInfectionViability assaysMolecular viabilityImportance of personPneumoniaReliable methodEpidemiologyPersons
2000
Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii
Maher N, Vermund S, Lasbury M, Lee C, Bartlett M, Unnasch T. Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii. Journal Of Clinical Microbiology 2000, 38: 1947-1952. PMID: 10790126, PMCID: PMC86630, DOI: 10.1128/jcm.38.5.1947-1952.2000.Peer-Reviewed Original ResearchConceptsPneumocystis carinii pneumoniaRespiratory specimensP. cariniiViable trophozoitesPatient respiratory specimensP. carinii DNAOpportunistic illnessesViability assaysCarinii pneumoniaPCP patientsShock protein 70 familyReverse transcription-PCRHeat shock protein 70 familyPneumocystis cariniiNoninvasive techniqueCarinii DNACariniiTranscription-PCREthidium bromide stainingOptimal assay conditionsTrophozoitesBromide stainingAssaysMolecular viabilitySpecific primers