2021
Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei
Toh JY, Nkouawa A, Sánchez SR, Shi H, Kolev NG, Tschudi C. Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei. Scientific Reports 2021, 11: 5755. PMID: 33707699, PMCID: PMC7952579, DOI: 10.1038/s41598-021-85225-2.Peer-Reviewed Original ResearchConceptsGene expression regulatory networksMetacyclic variant surface glycoproteinSingle RNA-binding proteinExpression regulatory networksRNA-binding proteinTsetse fly vectorBloodstream form trypanosomesAlanine-rich proteinPutative nucleic acidRNA-seq dataSubset of transcriptsCell linesDevelopmental progressionVariant surface glycoproteinMitochondrial genomeOverexpression cell linesFunction phenotypesRegulatory networksProcyclic trypanosomesTrypanosoma bruceiNegative regulatorRich proteinImportant humanMetacyclic parasitesGenes
2017
The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion
Christiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Molecular Microbiology 2017, 106: 74-92. PMID: 28742275, PMCID: PMC5607103, DOI: 10.1111/mmi.13754.Peer-Reviewed Original ResearchConceptsInfectious metacyclic formsTsetse fly vectorMetacyclic formsVariant surface glycoprotein expression sitesQuiescent cellsGene expression profilesFly vectorsAvailability of nutrientsMammalian invasionsCell surface componentsCell divisionProcyclic trypanosomesMammalian hostsTrypanosoma bruceiMRNA sequencingExpression profilesExpression sitesMetabolic enzymesBloodstream formsInfectious metacyclicsProtein 6TranscriptomeTsetse fliesProteomeProtein levels