2019
Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa
Koelmel J, Ulmer C, Fogelson S, Jones C, Botha H, Bangma J, Guillette T, Luus-Powell W, Sara J, Smit W, Albert K, Miller H, Guillette M, Olsen B, Cochran J, Garrett T, Yost R, Bowden J. Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa. Metabolomics 2019, 15: 38. PMID: 30838461, PMCID: PMC11005104, DOI: 10.1007/s11306-019-1490-9.Peer-Reviewed Original ResearchConceptsApplication of lipidomicsDiverse biological rolesWildlife studiesPossible disease mechanismsClasses of lipidsHigh-resolution tandem mass spectrometryLoskop DamBiological roleMozambique tilapiaCell deathMortality eventsPansteatitisLipidomicsOxidative damageDisease mechanismsAdipose tissuePromising biomarker candidatesBiomarker discoveryDisease etiologyTilapiaTandem mass spectrometryCeramideAquatic lifeLipidomeBiomarker candidatesChronic maternal cortisol excess during late gestation leads to metabolic alterations in the newborn heart
Walejko J, Antolic A, Koelmel J, Garrett T, Edison A, Keller-Wood M. Chronic maternal cortisol excess during late gestation leads to metabolic alterations in the newborn heart. AJP Endocrinology And Metabolism 2019, 316: e546-e556. PMID: 30620638, PMCID: PMC6459297, DOI: 10.1152/ajpendo.00386.2018.Peer-Reviewed Original ResearchConceptsNewborn heartLate gestationCortisol altersMaternal cortisolCardiac arrhythmiasFetal cardiac arrhythmiasMaternal serum specimensCortisol excessFetal exposureMaternal serumFetal metabolismCardiac metabolitesAbnormal elevationExcess cortisolLipid profileChronic increaseFetal serumGestation leadMetabolic alterationsSerum specimensHeart specimensFetal heartLipid metabolitesGlycerophospholipid metabolismCortisol
2018
Multiomics approach reveals metabolic changes in the heart at birth
Walejko J, Koelmel J, Garrett T, Edison A, Keller-Wood M. Multiomics approach reveals metabolic changes in the heart at birth. AJP Endocrinology And Metabolism 2018, 315: e1212-e1223. PMID: 30300011, PMCID: PMC6336953, DOI: 10.1152/ajpendo.00297.2018.Peer-Reviewed Original ResearchConceptsEnrichment analysisOverrepresentation enrichment analysisNewborn heartFetal heartTranscriptomic approachTranscriptomic levelT-testMetabolic changesFatty acidsMetabolomic levelsGlycerophospholipid metabolic pathwayTranscriptomic findingsMultiomics approachPropanoate metabolismTreatment of neonatesOxidative phosphorylationDifferential expressionDay of birthSingle-sample geneMetabolic pathwaysGreater abundanceStudent's t-testHeart failureRight ventricleIntraventricular septumExamining heat treatment for stabilization of the lipidome
Koelmel J, Jones C, Ulmer C, Garrett T, Yost R, Schock T, Bowden J. Examining heat treatment for stabilization of the lipidome. Bioanalysis 2018, 10: 291-305. PMID: 29451398, DOI: 10.4155/bio-2017-0209.Peer-Reviewed Original ResearchConceptsLiquid chromatography-high resolution tandem mass spectrometryEnzymatic productResolution tandem mass spectrometryHigh-resolution tandem mass spectrometryLipid biomarker researchTandem mass spectrometryLipid-based biomarkersEnzyme stabilizerMass spectrometryLipid identificationHeat treatmentSample weighingFlash freezingHeat-treated samplesFlash-frozen samplesMass Spectrometric Methodologies for Investigating the Metabolic Signatures of Parkinson’s Disease: Current Progress and Future Perspectives
Gill E, Koelmel J, Yost R, Okun M, Vedam-Mai V, Garrett T. Mass Spectrometric Methodologies for Investigating the Metabolic Signatures of Parkinson’s Disease: Current Progress and Future Perspectives. Analytical Chemistry 2018, 90: 2979-2986. PMID: 29384654, DOI: 10.1021/acs.analchem.7b04084.Peer-Reviewed Original ResearchConceptsParkinson's diseaseMetabolic signaturesContext of PDDegeneration of motorProdromal Parkinson's diseaseDisease-modifying therapiesBasal ganglia circuitryMotor deficitsDopaminergic neuronsDopamine metabolitesSubstantia nigraDisease progressionDefinitive biomarkersEarly diagnosisUseful biomarkerNeurodegenerative disordersDiseaseNonmotor componentsField of MSBiomarkersPreanalytical factorsMetabolomics studiesCellular pathwaysFuture perspectivesEarly stages
2017
Tissue distribution of perfluoroalkyl acids and health status in wild Mozambique tilapia (Oreochromis mossambicus) from Loskop Dam, Mpumalanga, South Africa
Bangma J, Reiner J, Botha H, Cantu T, Gouws M, Guillette M, Koelmel J, Luus-Powell W, Myburgh J, Rynders O, Sara J, Smit W, Bowden J. Tissue distribution of perfluoroalkyl acids and health status in wild Mozambique tilapia (Oreochromis mossambicus) from Loskop Dam, Mpumalanga, South Africa. Journal Of Environmental Sciences 2017, 61: 59-67. PMID: 29191316, PMCID: PMC6584947, DOI: 10.1016/j.jes.2017.03.041.Peer-Reviewed Original ResearchConceptsHealth statusInflammatory diseasesFish health statusAdipose tissueHealthy tilapiaTissue distributionTissue concentrationsPerfluoroalkyl acidsMale Mozambique tilapiaKidneyPerfluorooctanoic acidTissueBurdenLiverHigher PFAA concentrationsPerfluorooctanesulfonic acidStatusOrgansMozambique tilapiaPFAA concentrationsStatistical analysisSpleenIncidenceDiseaseCare