2018
Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions
Gathungu R, Larrea P, Sniatynski M, Marur V, Bowden J, Koelmel J, Starke-Reed P, Hubbard V, Kristal B. Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions. Analytical Chemistry 2018, 90: 13523-13532. PMID: 30265528, PMCID: PMC6297073, DOI: 10.1021/acs.analchem.8b03436.Peer-Reviewed Original ResearchConceptsSource fragmentsIonization source parametersSource fragmentationPrecursor ionsComplex samplesHeated electrospray ionization sourceElectrospray ionization sourceNegative ion modeIon modeTrace lipidsIonization sourceChromatographic separationLipid standardsComplex matricesShotgun lipidomicsIonsInterlaboratory comparison exerciseRelevant lipidsAppropriate source conditionsIdentical massLipidomicsAbundant massBiological relevancePotential misannotationsExactive
2017
Expanding Lipidome Coverage Using LC-MS/MS Data-Dependent Acquisition with Automated Exclusion List Generation
Koelmel J, Kroeger N, Gill E, Ulmer C, Bowden J, Patterson R, Yost R, Garrett T. Expanding Lipidome Coverage Using LC-MS/MS Data-Dependent Acquisition with Automated Exclusion List Generation. Journal Of The American Society For Mass Spectrometry 2017, 28: 908-917. PMID: 28265968, PMCID: PMC5408749, DOI: 10.1007/s13361-017-1608-0.Peer-Reviewed Original ResearchHR-MS/MS spectraUltra-high performance liquid chromatographyResolution tandem mass spectrometryHigh-resolution tandem mass spectrometryPositive ion modeField of lipidomicsTandem mass spectrometryMS/MSIon modePerformance liquid chromatographyMS spectraLC-MS/MS dataMass spectrometryChromatographic elutionSequential injectionLiquid chromatographyTrace speciesMS dataUnique precursorDependent acquisitionBiomoleculesBiological systemsNumber of precursors