Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions
Gathungu R, Larrea P, Sniatynski M, Marur V, Bowden J, Koelmel J, Starke-Reed P, Hubbard V, Kristal B. Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions. Analytical Chemistry 2018, 90: 13523-13532. PMID: 30265528, PMCID: PMC6297073, DOI: 10.1021/acs.analchem.8b03436.Peer-Reviewed Original ResearchConceptsSource fragmentsIonization source parametersSource fragmentationPrecursor ionsComplex samplesHeated electrospray ionization sourceElectrospray ionization sourceNegative ion modeIon modeTrace lipidsIonization sourceChromatographic separationLipid standardsComplex matricesShotgun lipidomicsIonsInterlaboratory comparison exerciseRelevant lipidsAppropriate source conditionsIdentical massLipidomicsAbundant massBiological relevancePotential misannotationsExactive