In vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes
Boucher P, Hines R. In vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes. Carcinogenesis 1995, 16: 383-392. PMID: 7859371, DOI: 10.1093/carcin/16.2.383.Peer-Reviewed Original ResearchConceptsNegative regulatory elementSpecific DNA-protein complexesDNA-protein complexesTransient expression assaysRegulatory elementsNuclear proteinsMouse fragmentsHeterologous promoterExpression assaysCell linesElectrophoretic mobility shift assaysMouse nuclear proteinMouse CYP1A1 geneSouthwestern blot analysisMobility shift assaysCYP1A1 geneNegative regulatory activitySimilar protection patternMouse hepatoma cell lineRodent cell linesCytosine-rich sequencesCytosine-rich regionsHuman CYP1A1 geneOrthologous sequencesRodent genes