2019
Integrative Activity of Mating Loci, Environmentally Responsive Genes, and Secondary Metabolism Pathways during Sexual Development of Chaetomium globosum
Wang Z, López-Giráldez F, Wang J, Trail F, Townsend JP. Integrative Activity of Mating Loci, Environmentally Responsive Genes, and Secondary Metabolism Pathways during Sexual Development of Chaetomium globosum. MBio 2019, 10: 10.1128/mbio.02119-19. PMID: 31822585, PMCID: PMC6904875, DOI: 10.1128/mbio.02119-19.Peer-Reviewed Original ResearchConceptsGene expressionMating lociFungal diversityResponsive genesSexual developmentSecondary metabolism gene clustersGenome-wide gene expressionMating-type genesRich fungal diversitySecondary metabolism pathwaysModel filamentous fungusSecondary metabolic pathwaysWide gene expressionNumerous secondary metabolitesGene expression responsesHistidine kinaseLife cycleSexual reproductionHomothallic speciesDevelopmental genesEvolutionary biologistsHeterokaryon incompatibilityAsexual reproductionDistinct morphological stagesEvolutionary history
2017
The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi
Trail F, Wang Z, Stefanko K, Cubba C, Townsend JP. The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi. PLOS Genetics 2017, 13: e1006867. PMID: 28704372, PMCID: PMC5509106, DOI: 10.1371/journal.pgen.1006867.Peer-Reviewed Original ResearchConceptsGene expressionFilamentous fungiCommon garden environmentBody developmentWide gene expressionGene expression phenotypesGene deletion studiesGene expression measurementsMulticellular developmentDivergent speciesSexual phenotypeGene familyEvolved increasesDivergent morphologyKnockout phenotypesAncestral levelsWhole genomeGarden environmentExpression phenotypesDeletion studiesRelevant genesPhenotypic analysisDevelopmental stagesExpression measurementsBody morphology
2016
PhyInformR: phylogenetic experimental design and phylogenomic data exploration in R
Dornburg A, Fisk JN, Tamagnan J, Townsend JP. PhyInformR: phylogenetic experimental design and phylogenomic data exploration in R. BMC Ecology And Evolution 2016, 16: 262. PMID: 27905871, PMCID: PMC5134231, DOI: 10.1186/s12862-016-0837-3.Peer-Reviewed Original ResearchConceptsParallel processingOpen-source software packageBayesian posterior distributionLack of softwareOpen-source programPhylogenetic experimental designProbability of resolutionUser hardwareData explorationData visualizationNext-generation sequence datasetsInformation contentSource programNovel visualizationPosterior distributionBayesian settingAdditional sample dataPower usersDataset partitionsBayesian approachSequence data setsSoftware packageDatasetGenomic information contentSoftware
2015
A comprehensive phylogeny of birds (Aves) using targeted next-generation DNA sequencing
Prum RO, Berv JS, Dornburg A, Field DJ, Townsend JP, Lemmon EM, Lemmon AR. A comprehensive phylogeny of birds (Aves) using targeted next-generation DNA sequencing. Nature 2015, 526: 569-573. PMID: 26444237, DOI: 10.1038/nature15697.Peer-Reviewed Original ResearchUtility of characters evolving at diverse rates of evolution to resolve quartet trees with unequal branch lengths: analytical predictions of long-branch effects
Su Z, Townsend JP. Utility of characters evolving at diverse rates of evolution to resolve quartet trees with unequal branch lengths: analytical predictions of long-branch effects. BMC Ecology And Evolution 2015, 15: 86. PMID: 25968460, PMCID: PMC4429678, DOI: 10.1186/s12862-015-0364-7.Peer-Reviewed Original ResearchMeSH KeywordsBayes TheoremBiological EvolutionComputer SimulationLikelihood FunctionsModels, GeneticPhylogenyConceptsGeneralized signalCorrect treeAnalytical predictionsNoise analysisDifferent inference methodsBayesian approachLength spaceInference methodsQuartet treesMaximum likelihoodSimulation studyBranch lengthsInferenceModel treePhylogenetic inferenceUtility of charactersAsymmetrical topologyLong-branch effectsConvergenceTopologyPredictionSpaceSignalsEvolutionH-CLAP: hierarchical clustering within a linear array with an application in genetics
Ghosh S, Townsend JP. H-CLAP: hierarchical clustering within a linear array with an application in genetics. Statistical Applications In Genetics And Molecular Biology 2015, 14: 125-141. PMID: 25803088, DOI: 10.1515/sagmb-2013-0076.Peer-Reviewed Original Research
2012
Response to: The relative utility of sequence divergence and phylogenetic informativeness profiling in phylogenetic study design
Moeller AH, Townsend JP. Response to: The relative utility of sequence divergence and phylogenetic informativeness profiling in phylogenetic study design. Molecular Phylogenetics And Evolution 2012, 66: 436. PMID: 23063589, DOI: 10.1016/j.ympev.2012.09.035.Commentaries, Editorials and Letters
2011
Phylogenetic informativeness profiling of 12 genes for 28 vertebrate taxa without divergence dates
Moeller AH, Townsend JP. Phylogenetic informativeness profiling of 12 genes for 28 vertebrate taxa without divergence dates. Molecular Phylogenetics And Evolution 2011, 60: 271-272. PMID: 21558010, DOI: 10.1016/j.ympev.2011.04.023.Peer-Reviewed Original ResearchGetting the Most Out of Your Fungal Microarray Data: Two Cost- and Time-Effective Methods
Mathioni SM, Beló A, Townsend JP, Donofrio NM. Getting the Most Out of Your Fungal Microarray Data: Two Cost- and Time-Effective Methods. Methods In Molecular Biology 2011, 722: 61-77. PMID: 21590413, DOI: 10.1007/978-1-61779-040-9_5.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsExpensive softwareData generationMultiple platformsGenomic data generationEfficient waySuch dataPossible informationGenome sequencing technologiesKind of analysisData analysisFungal dataUsersDifferent environmentsMicroarray dataBioinformaticistsCostExperimental costSoftwareProtocolBottleneckPlatformTaskTechnologyDataAcademia
2009
Evolution of Reproductive Morphology in Leaf Endophytes
Wang Z, Johnston PR, Yang ZL, Townsend JP. Evolution of Reproductive Morphology in Leaf Endophytes. PLOS ONE 2009, 4: e4246. PMID: 19158947, PMCID: PMC2617777, DOI: 10.1371/journal.pone.0004246.Peer-Reviewed Original ResearchConceptsLeaf endophytesEcological roleDead host tissuesCommon developmental pathwayEndophytic lifestyleAsexual developmentSaprobic speciesReproductive structuresReproductive morphologySaprobic fungiFungal tissueFungal classificationDead woodEndophytic fungiDevelopmental pathwaysBody sizeFungal morphologySignificant divergenceLeotiomycetesFungiEndophytesHost tissuesProblematic groupRhytismatalesImportant role
2005
Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination
Kasuga T, Townsend JP, Tian C, Gilbert LB, Mannhaupt G, Taylor JW, Glass NL. Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination. Nucleic Acids Research 2005, 33: 6469-6485. PMID: 16287898, PMCID: PMC1283539, DOI: 10.1093/nar/gki953.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBayes TheoremBlotting, NorthernCluster AnalysisDatabases, Nucleic AcidDictyosteliumExpressed Sequence TagsGene Expression ProfilingGenes, FungalNeurospora crassaOligonucleotide Array Sequence AnalysisOligonucleotide ProbesPromoter Regions, GeneticReproducibility of ResultsRNA, MessengerSpores, FungalTranscription, GeneticUstilagoConceptsConidial germinationGene expression levelsNeurospora crassaGene expressionPhytopathogenic fungus Ustilago maydisSocial amoeba Dictyostelium discoideumFilamentous ascomycete speciesPutative regulatory componentFungus Ustilago maydisAmoeba Dictyostelium discoideumExpression levelsAscomycete speciesRibosomal biogenesisRelative gene expression levelsNovel genesTranscriptional programsUstilago maydisTranscriptional profilingDictyostelium discoideumFunctional predictionTranscriptional mechanismsRegulatory componentsFilamentous fungiExpression profilesMicroarray profilingA Bayesian method for analysing spotted microarray data
Meiklejohn CD, Townsend JP. A Bayesian method for analysing spotted microarray data. Briefings In Bioinformatics 2005, 6: 318-330. PMID: 16420731, DOI: 10.1093/bib/6.4.318.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2004
Resolution of large and small differences in gene expression using models for the Bayesian analysis of gene expression levels and spotted DNA microarrays
Townsend JP. Resolution of large and small differences in gene expression using models for the Bayesian analysis of gene expression levels and spotted DNA microarrays. BMC Bioinformatics 2004, 5: 54. PMID: 15128431, PMCID: PMC420235, DOI: 10.1186/1471-2105-5-54.Peer-Reviewed Original Research
2002
Bayesian analysis of gene expression levels: statistical quantification of relative mRNA level across multiple strains or treatments
Townsend JP, Hartl DL. Bayesian analysis of gene expression levels: statistical quantification of relative mRNA level across multiple strains or treatments. Genome Biology 2002, 3: research0071.1. PMID: 12537560, PMCID: PMC151173, DOI: 10.1186/gb-2002-3-12-research0071.Peer-Reviewed Original ResearchMeSH KeywordsBayes TheoremDNA-Binding ProteinsEthanolGene DeletionGene Expression ProfilingGene Expression Regulation, FungalGenotypeModels, GeneticNuclear ProteinsOligonucleotide Array Sequence AnalysisRNA, FungalRNA, MessengerSaccharomyces cerevisiae ProteinsSpecies SpecificityTranscription FactorsZincConceptsGene of interestExpression levelsGene expression levelsTwofold thresholdGene basisTranscriptional responseMicroarray comparisonsMinimal mediumExpression differencesBiological insightsMicroarray analysisEthanol shockMicroarray dataRelative mRNA levelsGenesMRNA levelsMultiple strainsBayesian analysisDevelopmental state