2022
Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys
Belitardo E, Nery N, Ticona J, Portilho MM, Mello IO, Ribeiro GS, Reis MG, Costa F, Cummings DAT, Ko AI, Fofana MO. Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys. Diagnostic Microbiology And Infectious Disease 2022, 104: 115807. PMID: 36162285, PMCID: PMC9428330, DOI: 10.1016/j.diagmicrobio.2022.115807.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 incidenceCommunity-based cohortPopulation-level immunitySARS-CoV-2Large-scale serosurveysELISA optical densityIgG titersIgG ELISAPandemic evolvesSerologic surveyTitersOptical density valuesPopulation-level changesSerosurveyImmunitySingle dilutionSerial dilutionsNODCohortIncidenceELISASerum
2003
Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
Lüdtke C, Coutinho M, Jouglard S, Moreira C, Fernandes C, Brod C, Haake D, Ko A, Dellagostin O, Aleixo J. Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira. Brazilian Journal Of Microbiology 2003, 34: 1-4. DOI: 10.1590/s1517-83822003000500001.Peer-Reviewed Original ResearchBALB/c miceMonoclonal antibodiesPathogenic LeptospiraSP2/OWhole-cell antigen preparationsIndirect ELISANon-pathogenic serovarsIgG2b isotypesC miceSpleen cellsAntigen preparationsMAb specificityDiagnostic testsPathogenic serovarsRecombinant LipL32Non-pathogenic LeptospiraMAbsLeptospiraELISAHybridoma productionAntibodiesLipL32SerovarsOuter membrane proteinsCells