2024
Growth characteristics of HCT116 xenografts lacking asparagine synthetase vary according to sex
Aladelokun O, Lu L, Zheng J, Yan H, Jain A, Gibson J, Khan S, Johnson C. Growth characteristics of HCT116 xenografts lacking asparagine synthetase vary according to sex. Human Genomics 2024, 18: 67. PMID: 38886847, PMCID: PMC11184737, DOI: 10.1186/s40246-024-00635-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAspartate-Ammonia LigaseCarbon-Nitrogen Ligases with Glutamine as Amide-N-DonorCell ProliferationColorectal NeoplasmsFemaleGene Expression Regulation, NeoplasticHCT116 CellsHeterograftsHumansMaleMiceReceptors, EstrogenReceptors, G-Protein-CoupledSex FactorsXenograft Model Antitumor AssaysConceptsFemale tumor-bearing miceFemale CRC patientsTumor-bearing miceCRC patientsTumor growthInferior survivalAssociated with inferior survivalMetabolic reprogrammingG protein-coupled estrogen receptorTriggering metabolic reprogrammingSustained tumor growthSuppressed tumor growthExpression of asparagine synthetaseCancer cell linesBackgroundSex-related differencesSurvival improvementImpact of sexFemale miceEstrogen receptorCancer growthTranslational relevanceRewiring of metabolic pathwaysCancer burdenMetabolic pathwaysAsparagine synthetaseSynergistic Immunoregulation: harnessing CircRNAs and PiRNAs to Amplify PD-1/PD-L1 Inhibition Therapy
Han R, Rao X, Zhou H, Lu L. Synergistic Immunoregulation: harnessing CircRNAs and PiRNAs to Amplify PD-1/PD-L1 Inhibition Therapy. International Journal Of Nanomedicine 2024, 19: 4803-4834. PMID: 38828205, PMCID: PMC11144010, DOI: 10.2147/ijn.s461289.Peer-Reviewed Original ResearchConceptsRegulate PD-L1 expressionPD-L1 expressionPD-1/PD-L1Inhibition therapySensitive to immune checkpoint inhibitorsEfficacy of cancer immunotherapyPD-1/PD-L1 inhibitorsImmune checkpoint inhibitorsAnti-cancer immunityEfficacy of monotherapyExploration of combination strategiesModulate immune responsesMRNA vaccine technologyCheckpoint inhibitorsCancer immunotherapyRNA-based therapiesTreatment strategiesImmunomodulatory effectsCancer therapyImmune responseTherapyCancer treatmentVaccine technologyCombination strategiesCancer
2023
tRFtarget 2.0: expanding the targetome landscape of transfer RNA-derived fragments
Li N, Yao S, Yu G, Lu L, Wang Z. tRFtarget 2.0: expanding the targetome landscape of transfer RNA-derived fragments. Nucleic Acids Research 2023, 52: d345-d350. PMID: 37811890, PMCID: PMC10767876, DOI: 10.1093/nar/gkad815.Peer-Reviewed Original Research
2022
Neurospora crassa is a potential source of anti-cancer agents against breast cancer
Han R, Yang H, Ling C, Lu L. Neurospora crassa is a potential source of anti-cancer agents against breast cancer. Breast Cancer 2022, 29: 1032-1041. PMID: 35881300, DOI: 10.1007/s12282-022-01383-9.Peer-Reviewed Original ResearchConceptsBreast cancerT-47DBreast cancer stem cellsBreast cancer cell linesBreast cancer invasivenessCancer stem cell-related genesStem cell-related genesTumor cell proliferationCancer stem cellsMouse model resultsAnti-tumor agentsCell-related genesAnti-cancer agentsCancer cell linesTumor growthCancer invasivenessCancer stemCell proliferationMCF-10ACell linesCancerInhibition rateStem cellsSpheroid formationCASP3 activityThe steroid hormone estriol (E3) regulates epigenetic programming of fetal mouse brain and reproductive tract
Zhou Y, Gu B, Brichant G, Singh JP, Yang H, Chang H, Zhao Y, Cheng C, Liu ZW, Alderman MH, Lu L, Yang X, Gao XB, Taylor HS. The steroid hormone estriol (E3) regulates epigenetic programming of fetal mouse brain and reproductive tract. BMC Biology 2022, 20: 93. PMID: 35491423, PMCID: PMC9059368, DOI: 10.1186/s12915-022-01293-4.Peer-Reviewed Original ResearchConceptsEstrogen receptorMaintenance of pregnancyFetal mouse brainReproductive systemE3 micePregnancy outcomesEstrogen actionEstrogen potencyPregnant miceEstrogen exposureEstrogen signalingFetal developmentMouse brainSteroid hormonesUnexpected functional rolesPregnancyReproductive tractHormone estriolBrainReceptorsExpression levelsFetusesExploratory behaviorMiceNovel mechanismValeric acid acts as a novel HDAC3 inhibitor against prostate cancer
Han R, Yang H, Li Y, Ling C, Lu L. Valeric acid acts as a novel HDAC3 inhibitor against prostate cancer. Medical Oncology 2022, 39: 213. PMID: 36175803, PMCID: PMC9522682, DOI: 10.1007/s12032-022-01814-9.Peer-Reviewed Original ResearchConceptsProstate cancer cellsProstate cancerHDAC3 inhibitorCancer cellsCancer-related deathAnti-cancer effectsVivo mouse modelAnti-cancer efficacyAnti-cancer activityMouse modelNew agentsSecond causeHDAC inhibitorsCancerNormal cellsInhibitorsValeric acidCellsCASP3 activityCulture systemDiseaseChemosensitizers
2020
tRFtarget: a database for transfer RNA-derived fragment targets
Li N, Shan N, Lu L, Wang Z. tRFtarget: a database for transfer RNA-derived fragment targets. Nucleic Acids Research 2020, 49: d254-d260. PMID: 33035346, PMCID: PMC7779015, DOI: 10.1093/nar/gkaa831.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase PairingBase SequenceCaenorhabditis elegansDatabases, Nucleic AcidDrosophila melanogasterGene OntologyHumansMiceMolecular Sequence AnnotationNucleic Acid ConformationNucleic Acid HybridizationRhodobacter sphaeroidesRNA, MessengerRNA, Small UntranslatedRNA, TransferSchizosaccharomycesThermodynamicsXenopusZebrafishConceptsTarget genesTransfer RNASmall non-coding RNAsGene Ontology annotationsNon-coding RNAsFunctional pathway analysisAccessible web-based databaseMolecular functionsOntology annotationsBiological functionsPathway analysisMolecular mechanismsPhysiological processesTarget predictionHuman diseasesGenesMRNA transcriptsRNAWeb-based databaseConvenient linkTRFImportant roleRNAhybridTargetIntaRNAImpaired hypocretin/orexin system alters responses to salient stimuli in obese male mice
Tan Y, Hang F, Liu ZW, Stoiljkovic M, Wu M, Tu Y, Han W, Lee AM, Kelley C, Hajos M, Lu L, de Lecea L, de Araujo I, Picciotto M, Horvath TL, Gao XB. Impaired hypocretin/orexin system alters responses to salient stimuli in obese male mice. Journal Of Clinical Investigation 2020, 130: 4985-4998. PMID: 32516139, PMCID: PMC7456212, DOI: 10.1172/jci130889.Peer-Reviewed Original ResearchConceptsHcrt cellsObese miceDiet-induced obese miceObese male miceExcessive energy intakeNeuropeptide hypocretin/orexinHypocretin/orexinHcrt neuronsMale miceHcrt systemClinical studiesCommon causeSynaptic transmissionObese animalsEnergy intakeAcute stressCognitive functionSalient stimuliAlters responsesExact mechanismMiceHomeostatic regulationNeuronal networksBehavioral changesNeuronsZinc supplement ameliorates phthalates-induced reproductive toxicity in male rats
Gao HT, Di QN, Qian LL, Lu L, Li RX, Cao WX, Xu Q. Zinc supplement ameliorates phthalates-induced reproductive toxicity in male rats. Chemosphere 2020, 246: 125828. PMID: 31927381, DOI: 10.1016/j.chemosphere.2020.125828.Peer-Reviewed Original ResearchConceptsZinc supplementsMale ratsReproductive toxicityReproductive functionProtein expressionSteroidogenic proteinsSub-chronic exposureMale reproductive functionMale reproductive toxicityPathological examinationRat testicular cellsRatsTesticular cellsLHHealth risksExposureSerumSupplementsUrineToxicityMixture exposureTestisAlterationsExpressionToxicity evaluation
2018
Chronic low-dose exposure of nonylphenol alters energy homeostasis in the reproductive system of female rats
Di QN, Cao WX, Xu R, Lu L, Xu Q, Wang XB. Chronic low-dose exposure of nonylphenol alters energy homeostasis in the reproductive system of female rats. Toxicology And Applied Pharmacology 2018, 348: 67-75. PMID: 29641977, DOI: 10.1016/j.taap.2018.04.007.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsCarnitine O-PalmitoyltransferaseEndocrine DisruptorsEndometrial HyperplasiaEndometriumEnergy MetabolismEstradiolFatty AcidsFemaleGlutathioneLiverMalondialdehydeMetabolomicsOogenesisOvaryOxidation-ReductionPhenolsPPAR gammaRats, Sprague-DawleyReproductionRisk AssessmentSuperoxide DismutaseConceptsFemale reproductive toxicityReproductive toxicityFemale ratsCarnitine palmitoyltransferase IEnergy homeostasisSerum 17β-estradiol levelsChronic low-dose exposurePeroxisome proliferator-activated receptor gammaImpaired fatty acid oxidationProliferator-activated receptor gammaReproductive systemHigh-dose exposureLow-dose exposureHuman exposure scenariosNP exposureFatty acid oxidationKey reproductive tissuesEndometrium hyperplasiaL-carnitineReceptor gammaPotential biomarkersComprehensive metabolomic studyEndocrine-disrupting chemicalsMetabolic profileDevelopmental toxicityCombined effects of simultaneous exposure to six phthalates and emulsifier glycerol monosterate on male reproductive system in rats
Gao HT, Xu R, Cao WX, Di QN, Li RX, Lu L, Xu Q, Yu SQ. Combined effects of simultaneous exposure to six phthalates and emulsifier glycerol monosterate on male reproductive system in rats. Toxicology And Applied Pharmacology 2018, 341: 87-97. PMID: 29366639, DOI: 10.1016/j.taap.2018.01.013.Peer-Reviewed Original ResearchConceptsRat reproductive systemFood emulsifier glycerin monostearateReproductive systemMale rat reproductive systemSteroidogenic proteinsMale reproductive toxicityMale reproductive systemTime-dependent mannerSerum testosteroneAndrogen metabolismMale ratsReproductive toxicityMorphological damageUbiquitous phthalate estersMetabonomic analysisDaily dietSimultaneous exposureToxic effectsRatsGlycerin monostearateMixPToxicityHormone biosynthesisExposureGavage
2017
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats
Gao HT, Xu R, Cao WX, Qian LL, Wang M, Lu L, Xu Q, Yu SQ. Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats. Food And Chemical Toxicology 2017, 101: 94-104. PMID: 28089693, DOI: 10.1016/j.fct.2017.01.011.Peer-Reviewed Original ResearchConceptsLong-term low-dose exposureMale reproductive toxicityLow-dose exposureReproductive toxicityMale rat reproductive systemSteroidogenic proteinsRat reproductive systemApoptosis-related proteinsPathological examinationRat testicular cellsMale ratsTesticular testosteroneCell cycle progressionRatsTesticular cellsReproductive systemTestosteroneToxicityExposureCycle progressionCell cycleIntegrated exposureButyl benzylToxicity evaluationMixPDisruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease
Wu J, Yang Y, Sun C, Sun S, Li Q, Yao Y, Fei F, Lu L, Chang Z, Zhang W, Wang X, Luo F. Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease. DNA And Cell Biology 2017, 36: 10-17. PMID: 28045567, DOI: 10.1089/dna.2016.3459.Peer-Reviewed Original ResearchConceptsGAA mRNAFrameshift mutationEnzymatic activityGene expression changesGAA geneSpecies-specific differencesPresence of autophagosomesGlycogen accumulationMutant zebrafishGAA enzymatic activityWT zebrafishZebrafish modelDays postfertilizationExpression changesZebrafishDifferent speciesPhenotypic changesAdult stageLysosomal glycogen accumulationGlycogen storage disease type IIStorage disease type IIGenesProtein levelsProtein expressionMutants
2016
The Steroidogenic Acute Regulatory Protein (StAR) Is Regulated by the H19/let-7 Axis
Men Y, Fan Y, Shen Y, Lu L, Kallen AN. The Steroidogenic Acute Regulatory Protein (StAR) Is Regulated by the H19/let-7 Axis. Endocrinology 2016, 158: 402-409. PMID: 27813675, PMCID: PMC5413078, DOI: 10.1210/en.2016-1340.Peer-Reviewed Original ResearchConceptsSteroidogenic acute regulatory proteinRegulatory proteinsAcute regulatory proteinPost-transcriptional levelRate-limiting stepMicroRNA let-7H19/letRegulation of reproductionMurine cell linesLet-7Overexpression of H19Long noncoding RNA H19Regulation of steroidogenesisCellular growthSpecific tissuesNovel mechanismMultiple rolesNovel targetRNA H19StAR expressionExpression variesCell linesRegulationH19ProteinFood Emulsifier Glycerin Monostearate Increases Internal Exposure Levels of Six Priority Controlled Phthalate Esters and Exacerbates Their Male Reproductive Toxicities in Rats
Gao HT, Xu R, Cao WX, Zhou X, Yan YH, Lu L, Xu Q, Shen Y. Food Emulsifier Glycerin Monostearate Increases Internal Exposure Levels of Six Priority Controlled Phthalate Esters and Exacerbates Their Male Reproductive Toxicities in Rats. PLOS ONE 2016, 11: e0161253. PMID: 27575856, PMCID: PMC5004918, DOI: 10.1371/journal.pone.0161253.Peer-Reviewed Original ResearchConceptsInternal exposure levelsReproductive toxicityExposure levelsMale ratsTestosterone levelsFood emulsifier glycerin monostearatePAE exposureHistopathology of testesMale reproductive toxicityDEHP groupRat urineUbiquitous phthalate estersRatsPhthalate monoestersHistopathologyInjuryToxicityTestisGlycerin monostearatePrimary metabolitesExposureUPLC-MSFood additivesGavageLevelsToxaphene levels in retail food from the Pearl River Delta area of South China and an assessment of dietary intake
Jiang Y, Liu Z, Wu D, Zhang J, Zhou J, Li S, Lu L, Lin X, Lu S, Peng J. Toxaphene levels in retail food from the Pearl River Delta area of South China and an assessment of dietary intake. Chemosphere 2016, 152: 318-327. PMID: 26991380, DOI: 10.1016/j.chemosphere.2016.02.121.Peer-Reviewed Original ResearchConceptsParlar 26Dietary intakeRiver Delta areaIsotope dilution high-resolution gas chromatography/high-resolution mass spectrometryPg/Delta areaMaximum residue limitsInternational literature dataBody weight/dayLivestock meatEuropean maximum residue limitsPoultry meatPoultry productsToxaphene levelsCharacteristic congenersWeight/dayPearl River Delta areaAverage daily intakeResidue limitsEstimated average daily intakeAdverse health effectsToxaphene contaminationRetail foodsDifferent food categoriesSouth China
2015
H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase
Zhou J, Yang L, Zhong T, Mueller M, Men Y, Zhang N, Xie J, Giang K, Chung H, Sun X, Lu L, Carmichael GG, Taylor HS, Huang Y. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. Nature Communications 2015, 6: 10221. PMID: 26687445, PMCID: PMC4703905, DOI: 10.1038/ncomms10221.Peer-Reviewed Original ResearchConceptsS-adenosylhomocysteine hydrolaseCellular componentsDNA methylation genomeGenome-wide methylation profilingOnly mammalian enzymeNumerous gene lociS-adenosylhomocysteineMode of regulationDiverse cellular componentsMethylation genomeMammalian developmentMethylation dynamicsIgf2-H19Mammalian enzymeRegulatory circuitsDNA methylationDependent methyltransferasesMethylation changesMethylation profilingPotent feedback inhibitorEpigenetic alterationsGene locusH19 lncRNAFeedback inhibitorS-adenosylmethionine
2014
Genome‐wide association discoveries of alcohol dependence
Zuo L, Lu L, Tan Y, Pan X, Cai Y, Wang X, Hong J, Zhong C, Wang F, Zhang X, Vanderlinden LA, Tabakoff B, Luo X. Genome‐wide association discoveries of alcohol dependence. American Journal On Addictions 2014, 23: 526-539. PMID: 25278008, PMCID: PMC4187224, DOI: 10.1111/j.1521-0391.2014.12147.x.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesGenome-wide levelPotential biological functionsGWAS samplesADH clusterGenome-wide association discoveryRisk variantsBiological functionsAlcohol dehydrogenase clusterWide significant associationsRobust risk locusCis-eQTLsRisk lociNrd1Association studiesKIAA0040PKNOX2RNA expressionImportant roleHTR7Replicable associationsIndividual samplesSERINC2Mouse brainVariants
2013
The Imprinted H19 LncRNA Antagonizes Let-7 MicroRNAs
Kallen AN, Zhou XB, Xu J, Qiao C, Ma J, Yan L, Lu L, Liu C, Yi JS, Zhang H, Min W, Bennett AM, Gregory RI, Ding Y, Huang Y. The Imprinted H19 LncRNA Antagonizes Let-7 MicroRNAs. Molecular Cell 2013, 52: 101-112. PMID: 24055342, PMCID: PMC3843377, DOI: 10.1016/j.molcel.2013.08.027.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCell DifferentiationComputational BiologyDatabases, GeneticGene Expression ProfilingGene Expression RegulationGenomic ImprintingGenotypeHEK293 CellsHuman Umbilical Vein Endothelial CellsHumansMiceMicroRNAsMuscle DevelopmentMyoblasts, SkeletalPhenotypeRibonucleoproteinsRNA InterferenceRNA, Long NoncodingTime FactorsTransfectionConceptsLet-7 familyWide transcriptome analysisHuman genetic disordersNoncanonical binding siteLet-7 microRNALet-7 overexpressionGene functionH19 depletionTranscriptome analysisMuscle differentiationMolecular spongeUnexpected modeImportant regulatorAdult muscleH19 knockdownRecent implicationMiR-675Physiological significanceMicroRNAsH19Binding sitesGenetic disordersOverexpressionImportant roleFetal tissues
2012
Omega-3 and omega-6 fatty acid intakes and endometrial cancer risk in a population-based case–control study
Arem H, Neuhouser ML, Irwin ML, Cartmel B, Lu L, Risch H, Mayne ST, Yu H. Omega-3 and omega-6 fatty acid intakes and endometrial cancer risk in a population-based case–control study. European Journal Of Nutrition 2012, 52: 1251-1260. PMID: 22915050, PMCID: PMC3548981, DOI: 10.1007/s00394-012-0436-z.Peer-Reviewed Original ResearchConceptsEndometrial cancer riskFatty acid intakeCancer riskEndometrial cancerAcid intakeDietary intakePopulation-based case-control studyFish oil supplement useUnconditional logistic regression methodsLong-chain omega-3 fatty acidsOmega-3 fatty acidsIncident cancer casesCase-control studyAge-matched controlsFatty acidsPopulation-based sampleTotal fish intakeSupplement useFish intakeExtreme quartilesProtective associationCancer casesHigh intakeReduced riskFatty acids EPA