An Engineered Rare Codon Device for Optimization of Metabolic Pathways
Wang Y, Li C, Khan M, Wang Y, Ruan Y, Zhao B, Zhang B, Ma X, Zhang K, Zhao X, Ye G, Guo X, Feng G, He L, Ma G. An Engineered Rare Codon Device for Optimization of Metabolic Pathways. Scientific Reports 2016, 6: 20608. PMID: 26852704, PMCID: PMC4745014, DOI: 10.1038/srep20608.Peer-Reviewed Original ResearchMeSH KeywordsAspartate-tRNA LigaseCodonEscherichia coliEscherichia coli ProteinsFatty Acid Synthase, Type IIFatty AcidsGenes, ReporterInteinsMetabolic EngineeringMetabolic Networks and PathwaysMutagenesisRNA, TransferConceptsRare codonsCognate tRNAMetabolic pathwaysExpression levelsProtein expressionTarget protein expressionAspartyl-tRNA synthetaseCodons AGGReporter geneRelevant enzymesCopy numberEscherichia coliCodonFatty acid yieldGenesSteady-state kineticsE. coliTRNAPathwayExpressionColiIntermediate levelsTwo-fold increaseSynthetaseAcid yield