Semiquantitative Real-Time PCR to Distinguish Pneumocystis Pneumonia from Colonization in a Heterogeneous Population of HIV-Negative Immunocompromised Patients
Grønseth S, Rogne T, Hannula R, Åsvold BO, Afset JE, Damås JK. Semiquantitative Real-Time PCR to Distinguish Pneumocystis Pneumonia from Colonization in a Heterogeneous Population of HIV-Negative Immunocompromised Patients. Microbiology Spectrum 2021, 9: 10.1128/spectrum.00026-21. PMID: 34346746, PMCID: PMC8552647, DOI: 10.1128/spectrum.00026-21.Peer-Reviewed Original ResearchConceptsSemiquantitative real-time PCRPneumocystis pneumoniaReal-time PCRCutoff valuePCP statusHigher fungal loadHematological malignanciesPneumocystis jiroveciiHIV-negative immunocompromised patientsHuman immunodeficiency virus (HIV) infectionLower respiratory tract specimensRisk of PCPSolid organ transplant recipientsFungal loadHIV Pneumocystis pneumoniaImmunodeficiency virus infectionOrgan transplant recipientsSolid organ recipientsHIV-negative individualsPCR-positive patientsSolid organ transplantationRespiratory tract specimensVulnerable patient populationLife-threatening infectionsAcute respiratory syndrome