2020
Antigen Discovery, Bioinformatics and Biological Characterization of Novel Immunodominant Babesia microti Antigens
Verma N, Puri A, Essuman E, Skelton R, Anantharaman V, Zheng H, White S, Gunalan K, Takeda K, Bajpai S, Lepore TJ, Krause PJ, Aravind L, Kumar S. Antigen Discovery, Bioinformatics and Biological Characterization of Novel Immunodominant Babesia microti Antigens. Scientific Reports 2020, 10: 9598. PMID: 32533024, PMCID: PMC7293334, DOI: 10.1038/s41598-020-66273-6.Peer-Reviewed Original ResearchConceptsPotential biological functionsBiological functionsCellular localization studiesB. microtiEvolutionary relationshipsDomain architectureBioinformatics analysisRecombinant proteinsExtracellular domainLocalization studiesPrimary causative agentUnrelated groupsE. coliBiological characterizationNovel assayIntraerythrocytic parasitesBabesia microtiDistinct classesProteinCausative agentAntigen discoveryGlobal public health impactB. microti antibodiesB. microti antigenTransfusion of blood
2018
BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection
Thekkiniath J, Mootien S, Lawres L, Perrin BA, Gewirtz M, Krause PJ, Williams S, Doggett J, Ledizet M, Mamoun C. BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00067-18. PMID: 30093394, PMCID: PMC6156295, DOI: 10.1128/jcm.00067-18.Peer-Reviewed Original ResearchConceptsHuman babesiosisBabesia microti infectionCapture enzyme-linked immunosorbent assayAntigen capture enzyme-linked immunosorbent assayAntigen capture assayEnzyme-linked immunosorbent assayZoonotic infectious diseaseAcute infectionBlood transfusionAsymptomatic infectionMicroti infectionReal-time PCRBlood supplyAnimal reservoir hostsDonor bloodEpidemiological surveyHuman patientsImmune systemSerological assaysImmunodominant antigensInfectionInfectious diseasesIntraerythrocytic protozoan parasitePatientsImmunosorbent assay
2007
ATOVAQUONE AND AZITHROMYCIN TREATMENT FOR BABESIOSIS IN AN INFANT
Raju M, Salazar JC, Leopold H, Krause PJ. ATOVAQUONE AND AZITHROMYCIN TREATMENT FOR BABESIOSIS IN AN INFANT. The Pediatric Infectious Disease Journal 2007, 26: 181-183. PMID: 17259886, DOI: 10.1097/01.inf.0000250622.11505.8f.Peer-Reviewed Original ResearchConceptsCyanotic heart diseaseBabesia microti infectionTreatment of childrenOral azithromycinThin blood smearsInitial presentationSevere anemiaHeart diseaseMicroti infectionPolymerase chain reactionHepatic enzymesBlood smearsInfantsIntraerythrocytic parasitesChain reactionTreatmentBabesiosisLaboratory testsFebrileThrombocytopeniaCyanoticAnemiaClindamycinSerologyAzithromycin
2001
Diagnosis of Babesiosis Using an Immunoblot Serologic Test
Ryan R, Krause P, Radolf J, Freeman K, Spielman A, Lenz R, Levin A. Diagnosis of Babesiosis Using an Immunoblot Serologic Test. MSphere 2001, 8: 1177-1180. PMID: 11687460, PMCID: PMC96246, DOI: 10.1128/cdli.8.6.1177-1180.2001.Peer-Reviewed Original ResearchConceptsB. microti antigenAntibody testHuman babesiosisB. microtiReactivity of seraDiagnostic antibody testsHuman granulocytic ehrlichiosisDiagnosis of babesiosisImmunoblot stripsRoutine clinical diagnosisSerologic testsAcute babesiosisIFA testLaboratory evidenceClinical diagnosisImmunoblot assayGranulocytic ehrlichiosisLyme diseaseAntigenSerumGI strainsInterpretive criteriaReactive bandsTest sensitivityHuman subjects
2000
Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti
Lodes M, Houghton R, Bruinsma E, Mohamath R, Reynolds L, Benson D, Krause P, Reed S, Persing D. Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti. Infection And Immunity 2000, 68: 2783-2790. PMID: 10768973, PMCID: PMC97488, DOI: 10.1128/iai.68.5.2783-2790.2000.Peer-Reviewed Original ResearchConceptsRelated antigensBabesia microtiSerological expression cloningB. microtiHumoral immune responseB. microti infectionClass of antigensSporozoite surface antigenPatient serum poolsWestern blot analysisBlood transfusionMicroti infectionImmune responseSurface antigenImmunodominant antigensAntigenHuman babesiosisMolecular testsGenomic expression libraryPlasmodium spCausative agentSerum poolsImmunoreactive antigensBlot analysisMicroti