2014
A Humanized Mouse Model of Autoimmune Insulitis
Milam A, Maher SE, Gibson JA, Lebastchi J, Wen L, Ruddle NH, Herold KC, Bothwell AL. A Humanized Mouse Model of Autoimmune Insulitis. Diabetes 2014, 63: 1712-1724. PMID: 24478396, PMCID: PMC3994947, DOI: 10.2337/db13-1141.Peer-Reviewed Original ResearchConceptsT cellsDiabetic donorsInsulin stainingMouse modelAntigen-pulsed cellsAutoantigen-derived peptidesNOD mouse modelHumanized mouse modelType 1 diabetesPancreatic β-cellsT cell linesHuman T cellsIslet infiltrationAutoimmune diabetesNOD-SCIDAutoimmune insulitisHuman diabetesDestructive infiltrationMouse isletsMechanism of inductionΒ-cellsDiabetesDiabetes researchDisease modelsInsulitis
2011
Impaired lymphatic contraction associated with immunosuppression
Liao S, Cheng G, Conner DA, Huang Y, Kucherlapati RS, Munn LL, Ruddle NH, Jain RK, Fukumura D, Padera TP. Impaired lymphatic contraction associated with immunosuppression. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 18784-18789. PMID: 22065738, PMCID: PMC3219138, DOI: 10.1073/pnas.1116152108.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseLymphatic contractionsLymphatic functionImmune responseAntigen-presenting cellsNitric oxide synthaseEffective immune responseUnique mouse modelLymphatic vessel contractionLymphatic vessel functionLymph nodesAutoreactive responsesMultiple sclerosisVessel contractionInflammatory conditionsInducible NOSOxide synthaseMouse modelLymphatic metastasisVessel functionLymphatic endothelial cellsNitric oxideEndothelial cellsIntravital imagingPotential mechanismsBlocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses
Motallebzadeh R, Rehakova S, Conlon TM, Win TS, Callaghan CJ, Goddard M, Bolton EM, Ruddle NH, Bradley JA, Pettigrew GJ. Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses. The FASEB Journal 2011, 26: 51-62. PMID: 21926237, DOI: 10.1096/fj.11-186973.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesBone MarrowCD4-Positive T-LymphocytesChoristomaChronic DiseaseGraft RejectionHeart TransplantationIsoantibodiesLymphoid TissueLymphotoxin beta ReceptorLymphotoxin-betaMiceMice, Inbred C57BLMice, KnockoutMyocardiumNeovascularization, PathologicRecombinant Fusion ProteinsSignal TransductionSpleenTransplantation, HomologousConceptsTertiary lymphoid organsCardiac allograftsHeart allograftsB cellsLymphotoxin β receptor (LTβR) signalingEctopic lymphoid tissueGerminal center activityLTβR-IgTLO formationPostoperative administrationAccelerated rejectionHumoral autoimmunityAlloimmune responseAutoantibody productionAutoantibody responseHumoral responseLymphoid organsLymphoid tissueLymphoid organogenesisEffector antibodiesMouse modelAllograftsTransplantationAutoantibodiesCells
2010
A yeast‐based recombinogenic targeting toolset for transgenic analysis of human disease genes
Bentley KL, Shashikant CS, Wang W, Ruddle NH, Ruddle FH. A yeast‐based recombinogenic targeting toolset for transgenic analysis of human disease genes. Annals Of The New York Academy Of Sciences 2010, 1207: e58-e68. PMID: 20961307, DOI: 10.1111/j.1749-6632.2010.05712.x.Peer-Reviewed Original ResearchConceptsPolycystic kidney disease 1Yeast-bacterial shuttle vectorHuman disease genesFunction of genesLarge insert DNABacterial artificial chromosomeGene of interestTransgenic analysisGenomic fragmentArtificial chromosomesDNA insertsDisease genesBiological processesShuttle vectorHuman diseasesGenesGene modificationClaspettesPClasperMouse modelValuable resourceTransgenic mouse modelTransgenic miceCritical insightsImmune system