2019
Genetically diverse Plasmodium falciparum infections, within-host competition and symptomatic malaria in humans
Sondo P, Derra K, Lefevre T, Diallo-Nakanabo S, Tarnagda Z, Zampa O, Kazienga A, Valea I, Sorgho H, Ouedraogo J, Guiguemde T, Tinto H. Genetically diverse Plasmodium falciparum infections, within-host competition and symptomatic malaria in humans. Scientific Reports 2019, 9: 127. PMID: 30644435, PMCID: PMC6333925, DOI: 10.1038/s41598-018-36493-y.Peer-Reviewed Original ResearchConceptsParasite densityMalaria symptomsHemoglobin levelsP. falciparum genetic diversityMixed infectionsMAD20 allelic familyMono-infected individualsLower hemoglobin levelsPlasmodium falciparum infectionAllelic familiesLow parasite densitiesP. falciparum isolatesMixed strain infectionsFC27 allelesSymptomatic malariaOlder patientsFalciparum infectionYounger patientsPharmacovigilance studyFalciparum isolatesHigh parasitaemiaP. falciparumPolymorphic antigensPatientsInfection
2018
Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso
Somé A, Bazié T, Zongo I, Yerbanga R, Nikiéma F, Neya C, Taho L, Ouédraogo J. Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso. Parasites & Vectors 2018, 11: 323. PMID: 29843783, PMCID: PMC5975679, DOI: 10.1186/s13071-018-2895-4.Peer-Reviewed Original ResearchConceptsSymptomatic malaria patientsPolymerase chain reactionMalaria patientsAllelic familiesBobo-DioulassoMAD20 allelic familyUrban health centersCause of morbidityMerozoite surface protein 1K1 allelic familySurface protein 1Plasmodium falciparum msp1P. malariaUncomplicated malariaFalciparum infectionResultsA totalHealth centersBlood samplesP. falciparumConclusionsOur studyBlood spotsMalaria parasite populationsAllele frequenciesPatientsBurkina Faso
2016
HRP2 and pLDH-Based Rapid Diagnostic Tests, Expert Microscopy, and PCR for Detection of Malaria Infection during Pregnancy and at Delivery in Areas of Varied Transmission: A Prospective Cohort Study in Burkina Faso and Uganda
Kyabayinze D, Zongo I, Cunningham J, Gatton M, Angutoko P, Ategeka J, Compaoré Y, Muehlenbachs A, Mulondo J, Nakalembe M, Somé F, Ouattara A, Rouamba N, Ouédraogo J, Hopkins H, Bell D. HRP2 and pLDH-Based Rapid Diagnostic Tests, Expert Microscopy, and PCR for Detection of Malaria Infection during Pregnancy and at Delivery in Areas of Varied Transmission: A Prospective Cohort Study in Burkina Faso and Uganda. PLOS ONE 2016, 11: e0156954. PMID: 27380525, PMCID: PMC4933335, DOI: 10.1371/journal.pone.0156954.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, ProtozoanBurkina FasoDiagnostic Tests, RoutineFemaleFollow-Up StudiesHost-Parasite InteractionsHumansInfant, NewbornL-Lactate DehydrogenaseMalaria, FalciparumMicroscopyPlasmodium falciparumPoint-of-Care SystemsPolymerase Chain ReactionPregnancyPregnancy Trimester, SecondPregnancy Trimester, ThirdPrenatal CareProspective StudiesProtozoan ProteinsReproducibility of ResultsSeasonsSensitivity and SpecificityUgandaYoung AdultConceptsPLDH rapid diagnostic testsRapid diagnostic testsHistidine-rich protein 2Screening testMulti-center prospective studyDiagnostic testsDifferent malaria transmission settingsTororo District HospitalIntermittent preventive treatmentProspective cohort studyLow-density infectionsPCR-positive womenMalaria transmission settingsAppropriate screening testsTest positivity rateTreatment of malariaAntenatal visitsCohort studySymptomatic womenExpert microscopyThird trimesterIntermittent screeningPregnant womenProspective studyMalaria infection
2012
Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data
Auburn S, Campino S, Miotto O, Djimde A, Zongo I, Manske M, Maslen G, Mangano V, Alcock D, MacInnis B, Rockett K, Clark T, Doumbo O, Ouédraogo J, Kwiatkowski D. Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data. PLOS ONE 2012, 7: e32891. PMID: 22393456, PMCID: PMC3290604, DOI: 10.1371/journal.pone.0032891.Peer-Reviewed Original ResearchConceptsHost diversityIllumina GA platformMultiplicity of infectionGenome-wide datasetsPopulation-level diversitySingle-molecule sequencing technologiesNext-generation sequence dataPlasmodium falciparum diversityMsp-1 locusGenetic divergenceSequence dataSequencing technologiesNumber of clonesLevel diversityNovel insightsDiversityP. falciparum fieldLociMSP-1PCRRelative proportionsNew statistical approachSNPsClonesReduced sensitivity
1996
Selected P. falciparum specific immune responses are maintained in AIDS adults in Burkina Faso
MIGOT F, OUEDRAOGO J, DIALLO J, ZAMPAN H, DUBOIS B, SCOTT‐FINNIGAN T, .SANOU P, DELORON P. Selected P. falciparum specific immune responses are maintained in AIDS adults in Burkina Faso. Parasite Immunology 1996, 18: 333-339. PMID: 9229386, DOI: 10.1046/j.1365-3024.1996.d01-116.x.Peer-Reviewed Original ResearchMeSH KeywordsAcquired Immunodeficiency SyndromeAdultAnimalsAntigens, ProtozoanBurkina FasoCase-Control StudiesCD4 Lymphocyte CountCD4-Positive T-LymphocytesCytokinesHIV-1HumansIn Vitro TechniquesInterferon-gammaInterleukin-2Interleukin-4Lymphocyte ActivationMalaria, FalciparumMiddle AgedPlasmodium falciparumConceptsImmune responseSchizont extractCytokine productionHIV-1Healthy subjectsHIV-1-positive subjectsSpecific human immune responsesCrude schizont extractNegative healthy subjectsPlasmodium falciparum malariaHIV-1 infectionIL-4 productionSpecific immune responseHuman immune responseWhole blood culturesIL-2 productionPresence of PHAMerozoite surface proteinAIDS adultsLymphocyte subsetsFalciparum malariaClinical AIDSAIDS patientsPositive subjectsMalarial antigens