Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection.
Kengne P, Ouédraogo J, Zampan H, Veas F, Guiguemdé T. Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection. Parassitologia 2003, 45: 27-31. PMID: 15270541.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAntimalarialsDNA Mutational AnalysisDNA, ProtozoanDrug ResistanceFolic Acid AntagonistsHumansMalaria, FalciparumMutation, MissenseParasitemiaPlasmodium falciparumPoint MutationPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthProguanilProtozoan ProteinsPyrimethamineTetrahydrofolate DehydrogenaseConceptsMutation-specific PCRCycloguanil resistanceBlood samplesResistance isolatesResistance mutationsPlasmodium falciparumMixed infectionsChemosensitivity analysisBloodWhole bloodParasite lysateRapid DNA extractionPCR productsRestriction endonuclease digestionPCRAmplified productsDry filter paperPCR technologyPoint mutationsEndonuclease digestionUndigested samplesPatientsInfectionPyrimethamineMutations