2024
Investigating the dose-dependency of the midgut escape barrier using a mechanistic model of within-mosquito dengue virus population dynamics
Johnson R, Stopard I, Byrne H, Armstrong P, Brackney D, Lambert B. Investigating the dose-dependency of the midgut escape barrier using a mechanistic model of within-mosquito dengue virus population dynamics. PLOS Pathogens 2024, 20: e1011975. PMID: 38557892, PMCID: PMC11008821, DOI: 10.1371/journal.ppat.1011975.Peer-Reviewed Original ResearchConceptsBasal lamina layersInfection dynamicsYellow fever virusEpidemics of severe diseaseMidgut escape barrierDengue virusVirus population dynamicsAmount of virusEstablishment of infectionDose-dependentlyMosquito feeding behaviorDEN virusesExtrinsic incubation periodFever virusMidgut epitheliumBlood feedingFeeding eventsMidgutViral infection dynamicsZika virusAedes aegyptiCourse of infectionEscape barrierAedes albopictusIncubation period
2021
Vector competence of human-biting ticks Ixodes scapularis, Amblyomma americanum and Dermacentor variabilis for Powassan virus
Sharma R, Cozens D, Armstrong P, Brackney D. Vector competence of human-biting ticks Ixodes scapularis, Amblyomma americanum and Dermacentor variabilis for Powassan virus. Parasites & Vectors 2021, 14: 466. PMID: 34503550, PMCID: PMC8427896, DOI: 10.1186/s13071-021-04974-1.Peer-Reviewed Original ResearchConceptsNaïve miceBALB/c micePublic health threatViremic miceC micePowassan virusIxodes sppIxodes scapularisInfection statusConclusionsOur findingsMiceDermacentor variabilisHealth threatPOWVTransmission fociTick speciesPotential roleInfected nymphsCompetent vectorsVector competenceEpidemiologyHuman riskFuture studiesResultsThe resultsVirusEffective control of the motile stages of Amblyomma americanum and reduced Ehrlichia spp. prevalence in adults via permethrin treatment of white-tailed deer in coastal Connecticut, USA
Williams S, Stafford K, Linske M, Brackney D, LaBonte A, Stuber H, Cozens D. Effective control of the motile stages of Amblyomma americanum and reduced Ehrlichia spp. prevalence in adults via permethrin treatment of white-tailed deer in coastal Connecticut, USA. Ticks And Tick-borne Diseases 2021, 12: 101675. PMID: 33529986, DOI: 10.1016/j.ttbdis.2021.101675.Peer-Reviewed Original ResearchA mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons
Ma Q, Srivastav SP, Gamez S, Dayama G, Feitosa-Suntheimer F, Patterson EI, Johnson RM, Matson EM, Gold AS, Brackney DE, Connor JH, Colpitts TM, Hughes GL, Rasgon JL, Nolan T, Akbari OS, Lau NC. A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons. Genome Research 2021, 31: gr.265157.120. PMID: 33419731, PMCID: PMC7919454, DOI: 10.1101/gr.265157.120.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsGenomic resourcesMosquito cell culturesSmall RNA regulatory pathwaysSmall RNA expression profilesPIWI pathway genesRNA regulatory pathwaysSatellite DNA repeatsSmall RNA profilesCapacity of mosquitoesRNA interference (RNAi) pathwayRNA expression profilesImportant mosquito speciesTransposon activityGenome integritySiRNA populationsDNA repeatsInterference pathwayCluster lociCell culturesEvolutionary dynamicsPathway genesRegulatory pathwaysMosquito cellsExpression profiles
2008
Aedes aegyptiuses RNA interference in defense against Sindbis virus infection
Campbell C, Keene K, Brackney D, Olson K, Blair C, Wilusz J, Foy B. Aedes aegyptiuses RNA interference in defense against Sindbis virus infection. BMC Microbiology 2008, 8: 47. PMID: 18366655, PMCID: PMC2278134, DOI: 10.1186/1471-2180-8-47.Peer-Reviewed Original ResearchConceptsVirus-dependent mannerAgo2 protein levelsVirus-specific siRNAsAnti-viral defense mechanismProtein levelsSINV infectionRNAi componentsAnti-viral defenseRNAi pathwayRNAi responseBlood-fed controlsSmall RNAsDsRNA injectionRecombinant Sindbis virusRNA interferenceTranscript levelsSindbis virus infectionSINV RNAMost populationsStrand RNAPathway activitySINV replicationRNAVirus interactionsDefense mechanisms