1994
The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases
Duboise S, Vannier-Santos M, Costa-Pinto D, Rivas L, Pan A, Traub-Cseko Y, De Souza W, McMahon-Pratt D. The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases. Molecular And Biochemical Parasitology 1994, 68: 119-132. PMID: 7891736, DOI: 10.1016/0166-6851(94)00157-x.Peer-Reviewed Original ResearchConceptsTerminal domainMature proteinaseFlagellar pocketCysteine proteinasesParasite cell divisionCOOH-terminal domainProtein sequence dataAmastigote cysteine proteinasesElectron microscopic immunolocalizationLow-level secretionCell divisionSequence dataPlasma membraneIntracellular targetingLeishmania pifanoiProteolytic processingPulse labelingAcid proteinase inhibitorsProteinase inhibitorsCultured amastigotesLevel secretionL. pifanoiExtension domainBiosynthesisEnzymatic processing
1993
Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
Traub-Cseko Y, Duboise M, Boukai L, McMahon-Pratt D. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Molecular And Biochemical Parasitology 1993, 57: 101-115. PMID: 8426606, DOI: 10.1016/0166-6851(93)90248-v.Peer-Reviewed Original ResearchConceptsC-terminal extensionCysteine proteinase geneComparative sequence analysisCysteine proteinasesActive site residuesAmastigote cysteine proteinasesMexicana complexChromosome locationGenomic arrangementDistinct genesCDNA sequenceSequence dataDNA sequencesTrypanosoma bruceiProtein sequencesBiological functionsProteinase geneSite residuesDegenerate primersSelective conservationLysosomal organellesGlycine residueGenomic copiesSequence analysisLeishmania mexicana complex