Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.
LeBowitz J, Coburn C, McMahon-Pratt D, Beverley S. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 9736-9740. PMID: 2124701, PMCID: PMC55248, DOI: 10.1073/pnas.87.24.9736.Peer-Reviewed Original ResearchConceptsExpression vectorCis-acting elementsTrypanosomatid protozoan parasitesRNA editingSurface antigen geneMolecular paradigmProcessing eventsParasite proteinsGenetic analysisLeishmania expression vectorExpression sitesPathogenic speciesNormal transcriptMembrane glycoproteinsEscherichia coliGenesImmunoblot analysisProtozoan parasiteProteinAntigen geneColony assayLeishmania majorFamily membersL. amazonensisKilobases