1989
Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein.
Pan A, McMahon-Pratt D. Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein. The Journal Of Immunology 1989, 143: 1001-8. PMID: 2501384, DOI: 10.4049/jimmunol.143.3.1001.Peer-Reviewed Original ResearchConceptsTissue culture-derived trypomastigotesTrypanosoma cruziCruzi amastigotesPreliminary sequence dataStage-specific componentsCulture-derived trypomastigotesStage-specific monoclonal antibodiesSingle protein moleculesMembrane-enriched preparationsAmino acid compositionSequence dataEpimastigote membranesHuman chagasic seraStage differentiationAxenic cultureMolecular characterizationT. cruzi amastigotesEpimastigotesProtein moleculesProteinChagas diseaseMonoclonal antibodiesLeishmania speciesAcid compositionChagasic sera
1988
Intracellular signaling transduction in the differentiation of Trypanosoma cruzi: role of cAMP.
Rangel-Aldao R, Triana F, Comach G, Abate T, Fernández V, McMahon-Pratt D. Intracellular signaling transduction in the differentiation of Trypanosoma cruzi: role of cAMP. Biological Research 1988, 21: 403-8. PMID: 2855697.Peer-Reviewed Original ResearchConceptsCell differentiationCAMP-dependent protein kinaseTrypanosoma cruziTypes of proteinsTransformation of epimastigotesProtein kinaseMetacyclic trypomastigotesRole of cAMPIntracellular cAMP levelsT. cruziReceptor proteinEpimastigotesCruziDifferentiationMajor bandProteinCAMP levelsPolyclonal antibodiesSpecific activityRII antibodyCrude homogenatesTransductionKinaseCAMPNovel type