1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1994
The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases
Duboise S, Vannier-Santos M, Costa-Pinto D, Rivas L, Pan A, Traub-Cseko Y, De Souza W, McMahon-Pratt D. The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases. Molecular And Biochemical Parasitology 1994, 68: 119-132. PMID: 7891736, DOI: 10.1016/0166-6851(94)00157-x.Peer-Reviewed Original ResearchConceptsTerminal domainMature proteinaseFlagellar pocketCysteine proteinasesParasite cell divisionCOOH-terminal domainProtein sequence dataAmastigote cysteine proteinasesElectron microscopic immunolocalizationLow-level secretionCell divisionSequence dataPlasma membraneIntracellular targetingLeishmania pifanoiProteolytic processingPulse labelingAcid proteinase inhibitorsProteinase inhibitorsCultured amastigotesLevel secretionL. pifanoiExtension domainBiosynthesisEnzymatic processing
1993
Developmental Life Cycle of Leishmania—Cultivation and Characterization of Cultured Extracellular Amastigotes1
PAN A, DUBOISE S, EPERON S, RIVAS L, HODGKINSON V, TRAUB‐CSEKO Y, MCMAHON‐PRATT D. Developmental Life Cycle of Leishmania—Cultivation and Characterization of Cultured Extracellular Amastigotes1. Journal Of Eukaryotic Microbiology 1993, 40: 213-223. PMID: 8461895, DOI: 10.1111/j.1550-7408.1993.tb04906.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsPlethora of proteinsCultured amastigotesDevelopmental life cycleDevelopmental responsesDevelopmental regulationAxenic cultureAxenic linesAmastigote stageBiochemical evidenceLife cycleInfected macrophagesOrganismsEnvironmental parametersLeishmania promastigotesAmastigotesDevelopmental investigationsLeishmaniaProteinParasitesRegulationBiochemistryLinesLeishmania amastigotesCharacterizationLeishmania organisms