2023
Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
Gu Y, Guberman-Pfeffer M, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey F, Batista V, Malvankar N. Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity. Nature Microbiology 2023, 8: 284-298. PMID: 36732469, PMCID: PMC9999484, DOI: 10.1038/s41564-022-01315-5.Peer-Reviewed Original ResearchConceptsNanowire assembliesHigh electron conductivityExtracellular electron transportNanowire networksExtracellular electron acceptorsG. sulfurreducensElectron conductivityCryogenic electron microscopy structureNanowiresHigh conductivityElectron microscopy structureSerine proteasesDifferent biochemical environmentsElectron transportMicroscopy structureDiverse speciesGeobacter speciesSulfurreducensConductivityImportant bacteriaCharge interactionsElectron acceptorBiochemical environmentSpeciesIdentifies mechanisms
2022
Protein nanowires with tunable functionality and programmable self-assembly using sequence-controlled synthesis
Shapiro DM, Mandava G, Yalcin SE, Arranz-Gibert P, Dahl PJ, Shipps C, Gu Y, Srikanth V, Salazar-Morales AI, O’Brien J, Vanderschuren K, Vu D, Batista VS, Malvankar NS, Isaacs FJ. Protein nanowires with tunable functionality and programmable self-assembly using sequence-controlled synthesis. Nature Communications 2022, 13: 829. PMID: 35149672, PMCID: PMC8837800, DOI: 10.1038/s41467-022-28206-x.Peer-Reviewed Original ResearchConceptsProtein nanowiresElectronic functionalityChemical-based synthesisConductive protein nanowiresSequence-controlled synthesisHigh electronic conductivityGold nanoparticlesSite-specific conjugationSynthetic chemistryTunable functionalityElectronic conductivityTunable propertiesAttractive biomaterialNonstandard amino acidsSynthetic biologyNanowiresBiomaterialsProtein materialSynthesisAtomic structureMost biomaterialsIncorporation of tryptophanFunctionalityConductivityNanoparticles
2020
Electric field stimulates production of highly conductive microbial OmcZ nanowires
Yalcin SE, O’Brien J, Gu Y, Reiss K, Yi SM, Jain R, Srikanth V, Dahl PJ, Huynh W, Vu D, Acharya A, Chaudhuri S, Varga T, Batista VS, Malvankar NS. Electric field stimulates production of highly conductive microbial OmcZ nanowires. Nature Chemical Biology 2020, 16: 1136-1142. PMID: 32807967, PMCID: PMC7502555, DOI: 10.1038/s41589-020-0623-9.Peer-Reviewed Original ResearchConceptsConductive protein nanowiresIndividual nanowiresProtein nanowiresElectronic functionalityNanowiresElectric fieldGeobacter sulfurreducens biofilmsHigh conductivityMost natural materialsHigh stiffnessElectrical signalsBidirectional interfaceElectronic systemsLiving materialsNatural materialsΠ stackingSpectroscopic studiesEnergy productionNanospectroscopyConductivityStiffnessConformational switchingΒ-sheetHeme groupSensing
2015
Electronic Conductivity in Living Biofilms: Physical Meaning, Mechanisms, and Measurement Methods
Malvankar N, Lovley D. Electronic Conductivity in Living Biofilms: Physical Meaning, Mechanisms, and Measurement Methods. 2015, 211-248. DOI: 10.1002/9781119097426.ch7.Peer-Reviewed Original ResearchElectronic conductivityLong-range electron conductionDc electronic conductivityAC impedance spectroscopyDirect conductivity measurementIonic conductivityImpedance spectroscopyFuel cellsMicrobial fuel cellsElectronic insulatorElectron acceptorElectron conductionMechanism of conductivityFour-probe methodConductivity measurementsConductive propertiesBiofilm conductivityLiving biofilmMicrobial nanowiresInexpensive feedstockC-type cytochromesConductivityGeobacter sulfurreducensConductive filmsElectron flow
2012
Biofilm conductivity is a decisive variable for high-current-density Geobacter sulfurreducens microbial fuel cells
Malvankar N, Tuominen M, Lovley D. Biofilm conductivity is a decisive variable for high-current-density Geobacter sulfurreducens microbial fuel cells. Energy & Environmental Science 2012, 5: 5790-5797. DOI: 10.1039/c2ee03388g.Peer-Reviewed Original ResearchMicrobial fuel cellsHigh current densityCurrent densityFuel cellsFuel cell architectureElectrochemical impedance spectroscopy measurementsAnode biofilmImpedance spectroscopy measurementsMFC performanceElectrode materialsCurrent productionCurrent outputConductivityOrganic wastePractical applicationsSpectroscopy measurementsCell architecture