2025
Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes
Awuoche E, Smallenberger G, Bruzzese D, Orfano A, Weiss B, Aksoy S. Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes. PLOS Pathogens 2025, 21: e1012692. PMID: 39888974, PMCID: PMC11819587, DOI: 10.1371/journal.ppat.1012692.Peer-Reviewed Original ResearchConceptsHost physiologyInsect stage procyclic formsPrevent gut colonizationHigh-throughput RNA sequencingHost-pathogen dynamicsImmunity-related genesInfluence of microbiotaReduced lipid availabilitySubgroup speciesPresence of SpiroplasmaLipid biosynthesisExpression regulationGut colonizationGut environmentMicrobiota compositionProcyclic formsSpiroplasma infectionEscherichia coliRNA sequencingLipid synthesisTrypanosoma brucei parasitesInfection processSpiroplasmaGenetic traitsAfrican trypanosomes
2021
Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis
Son JH, Weiss BL, Schneider DI, Dera KM, Gstöttenmayer F, Opiro R, Echodu R, Saarman NP, Attardo GM, Onyango M, Abdalla A, Aksoy S. Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis. PLOS Pathogens 2021, 17: e1009539. PMID: 34529715, PMCID: PMC8478229, DOI: 10.1371/journal.ppat.1009539.Peer-Reviewed Original ResearchConceptsReproductive fitnessSpiroplasma infectionSex-biased gene expressionHigh-throughput RNA sequencingReproductive physiologyIntrauterine larval developmentMale reproductive fitnessPathogenic African trypanosomesEndosymbiotic bacteriaFly resistanceTsetse fecundityFemale spermathecaFemale fecundityRNA sequencingLarval developmentSpiroplasmaGene expressionAfrican trypanosomesReproductive tissuesReproductive homeostasisTsetse hostHuman diseasesPopulation sizeProtective phenotypeLab lines
2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
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