Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays
Santolamazza F, Avellino P, Siciliano G, Yao F, Lombardo F, Ouédraogo J, Modiano D, Alano P, Mangano V. Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays. Malaria Journal 2017, 16: 468. PMID: 29149898, PMCID: PMC5693539, DOI: 10.1186/s12936-017-2118-z.Peer-Reviewed Original ResearchMeSH KeywordsBurkina FasoHumansMicroscopyPlasmodium falciparumPopulation DynamicsProtozoan ProteinsReal-Time Polymerase Chain ReactionConceptsRatio of maleFalciparum gametocytesEpidemiological surveyRTqPCR assaysFemale gametocytesLarge-scale epidemiological surveysP. falciparum gametocytesPlasmodium falciparum gametocytesRobust molecular assaySubmicroscopic infectionsGametocyte sex ratioPeripheral bloodBlood samplesEndemic areasNovel markerReal-time qPCRMalaria parasitesPfs25TaqMan assaysGametocytesMolecular assaysHuman subjectsTime qPCRMale gametocytesParasite sex ratio