Experimental study of the relationship between Plasmodium gametocyte density and infection success in mosquitoes; implications for the evaluation of malaria transmission-reducing interventions
Da D, Churcher T, Yerbanga R, Yaméogo B, Sangaré I, Ouedraogo J, Sinden R, Blagborough A, Cohuet A. Experimental study of the relationship between Plasmodium gametocyte density and infection success in mosquitoes; implications for the evaluation of malaria transmission-reducing interventions. Experimental Parasitology 2014, 149: 74-83. PMID: 25541384, DOI: 10.1016/j.exppara.2014.12.010.Peer-Reviewed Original ResearchConceptsGametocyte densityParasite densityBlood samplesInfected bloodMouse bloodInfectious blood samplesMembrane feeding assaysPlasmodium berghei infectionTransmission-reducing interventionsPlasmodium falciparum samplesBlood dilutionEvaluation of transmissionInfected mouse bloodBerghei infectionPlasmodium infectionMosquito infectivityAnopheles coluzzii mosquitoesInhibitory factorMonoclonal antibodiesBloodAccurate relative measuresInfectionUninfected bloodHost bloodColuzzii mosquitoesTransmission blocking activity of Azadirachta indica and Guiera senegalensis extracts on the sporogonic development of Plasmodium falciparum field isolates in Anopheles coluzzii mosquitoes
Yerbanga R, Lucantoni L, Ouédraogo R, Da D, Yao F, Yaméogo K, Churcher T, Lupidi G, Taglialatela-Scafati O, Gouagna L, Cohuet A, Christophides G, Ouédraogo J, Habluetzel A. Transmission blocking activity of Azadirachta indica and Guiera senegalensis extracts on the sporogonic development of Plasmodium falciparum field isolates in Anopheles coluzzii mosquitoes. Parasites & Vectors 2014, 7: 185. PMID: 24735564, PMCID: PMC3996177, DOI: 10.1186/1756-3305-7-185.Peer-Reviewed Original ResearchConceptsOocyst prevalencePlasmodium falciparum fieldMalaria control toolsLate-stage gametocytesAnopheles coluzzii femalesEvidence of transmissionPharmacological strategiesAntiplasmodial effectCombination treatmentAnopheles coluzzii mosquitoesAntimalarial drugsMalaria controlMalaria parasitesPlasmodium falciparumMembrane feedingMosquito midgutHerbal formulationsGuiera senegalensisEthyl acetate extractPlant extractsEthanol extractHuman hostColuzzii mosquitoesNeem tree Azadirachta indicaOocyst density