2025
Detection of Pneumococcal Carriage in Asymptomatic Healthcare Workers
Waghela P, Davis R, Campbell M, Datta R, Hislop M, Vega N, Wurst L, Yolda-Carr D, Couch L, Hernandez M, Grant L, Alexander-Parrish R, Arguedas A, Gessner B, Martinello R, Weinberger D, Wyllie A. Detection of Pneumococcal Carriage in Asymptomatic Healthcare Workers. Open Forum Infectious Diseases 2025, 12: ofaf008. PMID: 39917332, PMCID: PMC11800483, DOI: 10.1093/ofid/ofaf008.Peer-Reviewed Original ResearchHealthcare workersAsymptomatic healthcare workersPatient care assistantsYoung to middle-aged adultsSignificant demographic characteristicsPneumococcal carriageRegistered NursesCohort of healthcare workersCare assistantsConvenience sampleStudy participantsDetection of pneumococcal carriageRates of pneumococcal carriageMedical doctorsPediatric immunization programsDemographic characteristicsReservoir of pneumococciVaccine-targeted serotypesAdult populationHealthcareStudy populationIncreased riskPneumococcal carriage prevalenceExposure to respiratory pathogensColonized individuals
2024
A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva
Peno C, Lin T, Hislop M, Yolda-Carr D, Farjado K, York A, Pitzer V, Weinberger D, Bei A, Allicock O, Wyllie A. A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva. Microbiology Spectrum 2024, 12: e00591-24. PMID: 39028185, PMCID: PMC11370248, DOI: 10.1128/spectrum.00591-24.Peer-Reviewed Original ResearchDetection of pneumococciDetection of pneumococcal carriagePneumococcal carriageCarriage surveillanceLow-resource settingsChildren attending childcare centersCarriage of pneumococciDNA extractionSaliva samplesMolecular methodsCultural enrichmentImprove surveillance effortsQPCR-based protocolPneumococcal vaccineExtraction-free methodMolecular detectionNucleic acid extractionVaccination strategiesPneumococciCulture-enrichment methodExtraction-free protocolPurified DNASalivaPaired samplesCarriage
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